 |
Distribution of CCR-5 genotypes and HLA class I B alleles in HIV-1 infected and uninfected injecting drug users from Rio de Janeiro, Brazil
Presented by Sylvia Lopes Maia Teixeira, Brazil.
Teixeira S.L.M.1, Bastos F.I.2, Hacker M.A.2, Morgado M.G.1
1Laboratory of AIDS and Molecular Immunology, Department of Immunology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, FIOCRUZ, Rio de Janeiro, Brazil, 2Center for Information on Science and Technology, Department of Health Information, Oswaldo Cruz Foundation, FIOCRUZ, Rio de Janeiro, Brazil
Background: Host genetic factors play an important role in the dynamics of HIV epidemic, and have been considered in studies of differential susceptibility and resistance to HIV-1 infection. Several class I and II HLA alleles have been associated to the heterogeneity of HIV-1 infection susceptibility, as protective or risk factors for HIV-1 transmission. Moreover, a 32-base pair deletion in the HIV-1 CCR5 gene-coding region (CCR5D32 mutation) confers resistance to HIV-1 infection in homozygous individuals.
Methods: In the present study, DNA samples from HIV-1 infected and uninfected IDUs/ex-IDUs from Rio de Janeiro, Brazil, were PCR amplified in order to determine CCR5 genotypes based on the presence of the CCR5D32 mutation (48 samples from HIV-1 seropositive; 558 samples from HIV-1 seronegative IDUs/ex-IDUs) and typed for the HLA-B locus (48 samples from HIV-1 seropositive; 55 samples from HIV-1 seronegative IDUs/ex-IDUs), using commercial kits based on the line probe assay methodology, in an attempt to verify possible associations between these genetic factors and susceptibility/resistance to HIV-1 infection in the IDU population.
Results: The distribution of CCR5 genotypes between the two IDU/ex-IDU HIV positive and negative groups did not differ (p-value=0.75). The homozygous mutant genotype D32/D32 was not found in this study. Although some differences in the HLA-B allele frequencies could be observed between the two IDU groups, none of these was statistically significant. The most frequent HLA-B alleles found in this study were B*15, B*35, B*51 and B*44.
Conclusions: Based on these results, we couldn’t infer the putative association between these genetic markers and susceptibility/resistance to HIV-1 infection in the IDU population here studied. This report can be considered as a pioneer descriptive study of the distribution of CCR5 genotypes and HLA-B alleles in a population from Rio de Janeiro, Brazil.
Back to the session -
Back to the Programme-at-a-Glance
|
|